Studies on interaction of 5 S RNA with ribosomal proteins

FEBS Letters

Bielka, H.²  Saarma, M.¹  Knespel, S.²  Kärgel, H.-J.²  Gross, B.²  Stahl, J.² 

[1] Institute of Chemical Physics and Biophysics, Academy of Sciences of the ESSR, Laboratory of Molecular Genetics, 200026 Tallinn, USSR;Central Institute of Molecular Biology, Academy of Sciences of the GDR, Department of Cell Physiology, Berlin-Buch, 1115, GDR

关键词: Ribosome;    Ribosomal protein;    RNA-protein interaction;    Protein blotting;    5 S RNA;    5 S RNA-binding protein;    TP 40;    total ribosomal protein from small subunits of rat liver ribosomes;    TP 60;    total protein from large subunits of rat liver ribosomes;    2-D PAGE;    two-dimensional polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(87)80889-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with ³²P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl₂) resulted in specific binding to a limited set of ribosomal proteins consisting of proteins L3, L4, L6, L13/15 and — to a lesser extent — L7 and L19. Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA.

【 授权许可】

Unknown