期刊论文详细信息
FEBS Letters | |
Studies on interaction of 5 S RNA with ribosomal proteins | |
Bielka, H.2  Saarma, M.1  Knespel, S.2  Kärgel, H.-J.2  Gross, B.2  Stahl, J.2  | |
[1] Institute of Chemical Physics and Biophysics, Academy of Sciences of the ESSR, Laboratory of Molecular Genetics, 200026 Tallinn, USSR;Central Institute of Molecular Biology, Academy of Sciences of the GDR, Department of Cell Physiology, Berlin-Buch, 1115, GDR | |
关键词: Ribosome; Ribosomal protein; RNA-protein interaction; Protein blotting; 5 S RNA; 5 S RNA-binding protein; TP 40; total ribosomal protein from small subunits of rat liver ribosomes; TP 60; total protein from large subunits of rat liver ribosomes; 2-D PAGE; two-dimensional polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(87)80889-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Proteins of the large ribosomal subunit of rat liver (TP 60) were immobilized by diffusion transfer onto nitrocellulose after two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Incubation of the TP 60 blots with 32P-labeled 5 S RNA under defined ionic conditions (300 mM KCl, 20 mM MgCl2) resulted in specific binding to a limited set of ribosomal proteins consisting of proteins L3, L4, L6, L13/15 and — to a lesser extent — L7 and L19. Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA.
【 授权许可】
Unknown
【 预 览 】
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RO201912020289555ZK.pdf | 267KB | download |