Placental alkaline phosphatase activity is inversely related to cell growth rate in HeLaS3 cervical cancer cells

FEBS Letters

Green, Christopher D.¹  Telfer, Joan F.¹ 

[1] Biochemistry Department, University of Liverpool, PO Box 147, Liverpool, L69 3BX, UK

关键词: Placental alkaline phosphatase;    Cell growth;    Epidermal growth factor;    Mimosine: Gl phase;    EGF;    epidermal growth factor;    FCS;    foetal calf serum;    MEM;    minimal essential medium;    PLAP;    placental alkaline phosphatase;   

DOI  :  10.1016/0014-5793(93)80229-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 cells. We found that PLAP inducers, Na-butyrate, dexamethasone, bromodeoxyuridine and dibutyryl cAMP caused a dose-dependent reduction in growth rate. Mimosine, an agent that blocks the cell cycle in Gl, caused an increase in PLAP activity whilst the mitogen EGF caused a corresponding decrease in PLAP activity. PLAP activity may therefore be related to cell proliferation rate.

【 授权许可】

Unknown