Structure of three acidic O‐linked carbohydrate chains of porcine zona pellucida glycoproteins

FEBS Letters

Damm, Jan B.L.²  Vliegenthart, Johannes F.G.¹  Hokke, Comelis H.¹  Kamerling, Johannis P.¹ 

[1] Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands;Organon International BV, Oss, The Netherlands

关键词: Porcine zona pellucida glycoprotein;    Sulfated N-acetyllactosamine;    O-Linked carbohydrate chain;    FPLC;    fast protein liquid chromatography;    HOHAHA;    homonuclear Hartmann-Hahn;    HPLC;    high-pressure liquid chromatography;    NAc;    N-acetyl;    Neu5Ac;    N-acetylneuraminic acid;    Neu5Gc;    N-glycolylneuraminic acid;    NGc;    N-glycolyl;    PNGase-F;    peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F;    pZP;    porcine zona pellucida;    ZP;    zona pellucida;    1D;    one dimensional;    2D;    two dimensional;   

DOI  :  10.1016/0014-5793(93)80186-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Structural analysis by ID and 2D ¹H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Galβl-4(6SO₄-)GlcNAcβl-3Galβl-4GlcNAcβ1-3Galβ1-3GalNAc-ol Neu5Gcα2-3Gal/91 -4(6SO₄-)GlcNAqβl -3Ga1βl -4GlcNAcβl -3Galβl -3GalNAc-ol Neu5Acα2-3Galβl -4(6SO₄-)GlcNAcβl -3Galβl -4GlcNAcβl -3Galβl-3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins.

【 授权许可】

Unknown