Separation of isoenzymes of citrate synthase and isocitrate dehydrogenase by fast protein liquid chromatography

FEBS Letters

Reeves, H.C.¹  O'Neil, S.¹  Weitzman, P.D.J.¹  Mitchell, C.G.¹ 

[1] Department of Biochemistry, University of Bath, Bath BA2 7AY, England

关键词: FPLC;    Isoenzyme separation Citrate synthase Isocitrate dehydrogenase (Pseudomonas aeruginosa;    Acinetobacter calcoaceticus);   

DOI  :  10.1016/0014-5793(86)80248-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fast protein liquid chromatography (FPLC) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of Pseudomonas aeruginosa and Acinetobacter calcoaceticus The advantages of FPLC over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution.

【 授权许可】

Unknown