Changes in the expression of guanine nucleotide‐binding proteins during differentiation of 3T3‐F442A cells in a hormonally defined medium

FEBS Letters

Kilgour, Elaine¹  Anderson, Neil G.¹ 

[1] The Hannah Research Institute, Ayr KA6 5HL, Scotland, UK

关键词: 3T3-F442A cell;    Adipocyte;    Differentiation;    G protein;    Adenylyl cyclase;    AC;    adenylyl cyclase;    PBS;    phosphate-buffered saline;    EGF;    epidermal growth factor;    DMEM;    Dulbecco's modified Eagle's medium;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid;    GPDH;    α-glycerophosphate dehydrogenase;   

DOI  :  10.1016/0014-5793(93)80942-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using specific antisera, the expression of the G protein α subunits, G₈, g_i1, G_i2, G_i3, and G₀, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two G_s isoforms and decreases in the expression of both G_i2 and G_i3. Differentiation also resulted in a 2- to 4-fold increase in forskolin-stimulated adenylyl cyclase activity and a 15-fold increase in the response of cells to a β-adrenergic agonist. The increase in G_s expression was also observed, to a lesser degree, in cells maintained at confluence under conditions where morphological conversion was negligible and the decreased expression of G_i2 and G_i3 and the increased β-adrenergic responsiveness did not occur.

【 授权许可】

Unknown