Stimulated decay of Superoxide caused by ferritin‐bound copper

FEBS Letters

Ulvik, Rune J.¹  Bolann, Bjørn J.¹ 

[1] Laboratory of Clinical Biochemistry, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway

关键词: Ferritin;    Superoxide;    Copper;    Catalysis;   

DOI  :  10.1016/0014-5793(93)80940-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The redox interaction between O₂ ⁻ and ferritin cannot solely be regarded as a Fe(II) release reaction. We demonstrate that native copper bound to horse spleen ferritin and apoferritin, stimulated the decay of O₂ ⁻ in a catalytic reaction. Copper was determined by atomic absorption spectrophotometry. Decay of O₂ ⁻ was monitored spectrophotometrically as the decrease in (A ₂₅₀-A ₃₆₀) at pH 9.5. The catalytic effect was linearly related to the copper content of the protein. Ferritin copper was less efficient than equimolar CuCl₂, and iron-poor ferritin was more efficient than iron-rich ferritin. The results support a direct antioxidant function of ferritin.

【 授权许可】

Unknown