Decay of Superoxide catalyzed by ferritin

FEBS Letters

Ulvik, Rune J.¹  Bolann, Bjørn J.¹ 

[1] Laboratory of Clinical Biochemistry, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway

关键词: Ferritin;    Superoxide;    Spectrophotometry;    Kinetics;    Catalysis;   

DOI  :  10.1016/0014-5793(93)80010-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ferritin iron can be reduced by O^.− ₂, released, and form a Fe(II)-chelator complex. However, the thermodynamic influence of the chelator may disturb the reaction balance. We therefore excluded the chelator and measured instead the effect of ferritin on the decay of O^.− ₂, monitored by direct spectrophotometry at pH 9.5. Ferritin, but not apoferritin, accelerated the decay of O⁻ ₂. Ferritin iron was apparently the responsible agent. The effect of ferritin was maintained after several bursts of O^.− ₂, and the ratio degraded O^.− ₂/released Fe(II) greatly exceeded one, consistent with a catalytic reaction.

【 授权许可】

Unknown