Characterization of a membrane‐associated, receptor and G‐protein responsive phosphoinositide‐specific phospholipase C from avian erythrocytes

FEBS Letters

Ruiz-Larrea, Fernanda¹  Berrie, Christopher P.² 

[1] National Centre of Biotechnology, Cantoblanco, 28049 Madrid, Spain;Department of Human Anat. and Cell Biology, University of Liverpool, Liverpool L69 3BX, UK

关键词: Phosphoinositide;    Phospholipase-C;    G-protein;    Turkey erythrocyte;    Cytoskeleton;    Purinergic receptor;   

DOI  :  10.1016/0014-5793(93)80988-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the reconstitution and purification of a membrane-associated phosphoinositide-specific phospholipase C (PIC) from turkey erythrocyte ghosts. This PIC is responsive to a G-protein coupled to P_2y purinergic receptors which are expressed in turkey erythrocytes. Reconstitution is achieved by adding partially purified PIC to [³H]inositol-prelabelled turkey erythrocyte membranes depleted of their endogenous PIC (acceptor membranes). PIC activity is associated with a 52 kDa polypeptide on SDS-polyacrylamide gel electrophoresis. Addition of a 307-fold purified enzyme to the acceptor membranes has no effect on basal PIC activity, but markedly increases the response to GTPγS and P_2y-purinergic receptor activation.

【 授权许可】

Unknown