Primary structures and expression from cDNAs of rat opioid receptor δ‐and μ‐subtypes

FEBS Letters

Takeshima, Hiroshi¹  Kato, Shigehisa²  Fukuda, Kazuhiko²  Nishi, Miyuki¹  Mori, Kenjiro² 

[1] International Institute for Advanced Studies, Shimadzu N-80, Nishinokyo-Kuwahara-cho, Kyoto 604, Japan;Department of Anesthesia, Kyoto University Hospital, Kyoto 606-01, Japan

关键词: Opioid receptor;    cDNA cloning;    cDNA expression;    RNA blot hybridization analysis;    Rat brain;    G-proteins;    guanine nucleotide-binding regulatory proteins;    CHO cells;    Chinese hamster ovary cells;    DADLE;    [d-Ala2;    d-Leu5]enkephalin;    DPDPE;    [d-penicillamine2;    d-penicillamine5]enkephalin;    DSLET;    [d-Ser2;    Leu5]enkephalin-Thr;    DAGO;    [d-Ala2;    MePhe4;    Glyol5]enkephalin;   

DOI  :  10.1016/0014-5793(93)81011-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequences of rat opioid receptors (designated as ROR-A and ROR-B) have been deduced by cloning and sequencing the cDNAs. The ligand-binding properties of ROR-A and ROR-B expressed from the cloned cDNAs in Chinese hamster ovary cells correspond most closely to those of the pharmacologically defined δ- and μ-opioid receptor subtypes, respectively. RNA blot hybridization analysis revealed that cerebrum and brainstem contain both ROR-A and ROR-B mRNAs, whereas neither ROR-A nor ROR-B mRNAs can be detected in cerebellum.

【 授权许可】

Unknown