Glycosyl‐phosphatidylinositol‐specific phospholipase D

FEBS Letters

Bolli, Reinhard¹  Brodbeck, Urs²  Hoener, Marius C.² 

[1] ZLB Central Laboratory, Blood Transfusion Services SRC, Bern, Switzerland;Institute of Biochemistry and Molecular Biology, University of Bern, Bern, Switzerland

关键词: Glycosyl-phosphatidylinositol;    Phospholipase D;    Acetylcholinesterase;    High-density lipoprotein;    Apolipoprotein A-I;    GPI;    glycosyl-phosphatidylinositol;    PLD;    phospholipase D;    HDL;    high-density lipoprotein;    apo;    apolipoprotein;    AChE;    acetylcholinesterase;    mf-AChE;    membrane-form of AChE;    s-AChE;    soluble AChE;    CMC;    critical micellar concentration;   

DOI  :  10.1016/0014-5793(93)80170-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycosyl-phosphatidylinositol-specific phospholipase D (GPI-PLD) is an amphiphilic protein which, in serum, is associated with high-density lipoproteins (HDL). It is shown that the major component of the HDL fraction, apolipoprotein A-I (apo A-I), is responsible for this association. In the absence of apo A-I, purified GPI-PLD occurred as virtually inactive aggregates which became disaggregated by apo A-I. The enzyme/apo A-I complex efficiently hydrolyzed the solubilized GPI-anchored substrate, acetylcholinesterase. Triton X-100 was also able to dissociate aggregated GPI-PLD, however, it strongly inhibited enzyme activity at detergent concentrations above the critical micellar concentration.

【 授权许可】

Unknown