Targeted delivery of hygromycin B using reconstituted Sendai viral envelopes lacking hemagglutinin‐neuraminidase

FEBS Letters

Bagai, Sangeeta¹  Sarkar, Debi P.¹ 

[1] Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India

关键词: Sendai;    Reconstitution;    Targeting;    Fusion;    Hygromycin B;    ASGP-R;    asialoglycoprotein receptor;    D-MEM;    Dulbecco's modified Eagle's medium;    D-PBS;    Dulbecco's phosphate buffered saline;    Tx100;    Triton X-100;    DTT;    dithiothreitol;    ID50;    inhibitory dose;    RBC;    red blood cell;    WGA;    wheat germ agglutinin;    poly(U);    polyuridylic acid;   

DOI  :  10.1016/0014-5793(93)81787-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hygromycin B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). Incubation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of hygromycin B to the cell cytoplasm, as was inferred from inhibition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated by the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, subsequently leading to fusion between the two membranes. The cytotoxic effect of hygromycin B enclosed in F-virosomes was comparable with that of F,HN-virosomes containing both hemagglutinin-neuraminidase (HN) and F protein and F,HN_red-virosomes containing HN whose disulfide bonds were irreversibly reduced (HN_red). Hygromycin B loaded fusogenic liposomes were prepared by coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to be more active than F-virosomes at the same fusion protein concentrations.

【 授权许可】

Unknown