FEBS Letters | |
Targeted delivery of hygromycin B using reconstituted Sendai viral envelopes lacking hemagglutinin‐neuraminidase | |
Bagai, Sangeeta1  Sarkar, Debi P.1  | |
[1] Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India | |
关键词: Sendai; Reconstitution; Targeting; Fusion; Hygromycin B; ASGP-R; asialoglycoprotein receptor; D-MEM; Dulbecco's modified Eagle's medium; D-PBS; Dulbecco's phosphate buffered saline; Tx100; Triton X-100; DTT; dithiothreitol; ID50; inhibitory dose; RBC; red blood cell; WGA; wheat germ agglutinin; poly(U); polyuridylic acid; | |
DOI : 10.1016/0014-5793(93)81787-Z | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Hygromycin B was encapsulated in reconstituted Sendai viral envelopes containing only the fusion (F) protein (F-virosomes). Incubation of loaded F-virosomes with cultured HepG2 cells resulted in fusion mediated delivery of hygromycin B to the cell cytoplasm, as was inferred from inhibition of DNA synthesis. Binding of the F-virosomes to HepG2 cells was mediated by the interaction of terminal β-galactose residues of fusion protein with asialoglycoprotein receptor on HepG2 cells, subsequently leading to fusion between the two membranes. The cytotoxic effect of hygromycin B enclosed in F-virosomes was comparable with that of F,HN-virosomes containing both hemagglutinin-neuraminidase (HN) and F protein and F,HNred-virosomes containing HN whose disulfide bonds were irreversibly reduced (HNred). Hygromycin B loaded fusogenic liposomes were prepared by coreconstituting the viral envelope containing only fusion protein with exogenous lipids. These fusogenic liposomes were found to be more active than F-virosomes at the same fusion protein concentrations.
【 授权许可】
Unknown
【 预 览 】
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