How is leghemoglobin involved in peribacteroid membrane degradation during nodule senescence?

FEBS Letters

Day, David A.¹  Moreau, Sophie²  Herrada, Gilles²  Puppo, Alain²  Rigaud, Jean² 

[1] Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra, ACT 2601, Australia;Laboratoire de Biologie Végétale et Microbiologie, URA CNRS 1114 Université de Nice — Sophia Antipolis, Parc Valrose, 06108 Nice Cedex 2, France

关键词: Peribacteroid membrane;    Senescence;    Lipid peroxidation;    Leghemoglobin;    Dicarboxylate transport;    French-bean nodule;    Symbiosome;    BSA;    bovine serum albumin;    DTT;    dithiothreitol;    Lb;    leghemoglobin;    PBM;    peribacteroid membrane;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(93)81755-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An increase in the rate of succinate and glutamate uptake by isolated symbiosomes from French bean nodules was observed in the presence of iron plus H₂O₂. The lipid bilayer, and not proteins involved in transport, seems to be the major target of radical attack. Leghemoglobin in the presence of a 6-fold excess of H₂O₂ (where heme breakdown and iron release occurred) provoked also an increase in peribacteroid membrane permeability. In contrast, this hemoprotein in the presence of a 2-fold excess of H₂O₂ (where a protein radical was generated) was without effect. We suggest that in vivo the release of heme iron may constitute the major process concerning the involvement of leghemoglobin in the degradation of the peribacteroid membrane during nodule senescence.

【 授权许可】

Unknown