A dicarboxylate transporter on the peribacteroid membrane of soybean nodules

FEBS Letters

Gresshoff, Peter M.³  Day, David A.²  Udvardi, Michael K.²  Price, G.Dean¹ 

[1] P.E.B. Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia;Botany Department, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia;Plant Molecular Genetics (OHLD), College of Agriculture, University of Tennessee, Knoxville, USA

关键词: Nitrogen fixation;    Peribacteroid membrane;    Dicarboxylate transporter;    (Glycine max (L.);    Bradyrhizobium japonicum);    PBM;    peribacteroid membrane;    PBUs;    peribacteroid units;    BM;    bacteroid inner membrane;    M;    malate;   

DOI  :  10.1016/0014-5793(88)80697-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using preparations of peribacteroid membrane (PBM)-enclosed bacteroids from soybean root nodules, we show here that the PBM possesses a dicarboxylate transporter capable of mediating a rapid flux of dicarboxylate anions, such as malate and succinate, to the bacteroids inside the nodule. The transporter has a higher affinity for the monovalent malate anion than for the succinate anion (K _m = 2 and 15 μM, respectively) although the V _max for malate⁻ appears to be lower than for succinate⁻ (V _max = 11 and 30 nmol·min⁻¹·mg protein⁻¹, respectively).

【 授权许可】

Unknown