FEBS Letters | |
Cytochrome P‐450: hexameric structure of the purified LM4 form | |
Myasoedova, K.N.1  Tsuprun, V.L.2  | |
[1] N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow 117977, Russian Federation;A.V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Moscow 117333, Russian Federation | |
关键词: Cytochrome P-450LM4; Quaternary structure; Hexamer; Immobilized protein; | |
DOI : 10.1016/0014-5793(93)81083-C | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Purified cytochrome P-450LM4 was found to be monodisperse in 20% glycerol by analytical ultracentrifugation. Its S 20,w value was quite similar to that of hexameric P-450LM2. At lower glycerol concentrations the P-450LM4 oligomers showed a tendency to aggregate. The P-450LM4 oligomers were immobilized on Ultrogel A4 under conditions allowing only one covalent link to the matrix per oligomer. In the presence of SDS, the oligomers dissociated leaving only 1/6th of the initial amount of bound protein on the matrix, suggesting that the purified P-450LM4 is a hexamer. This was confirmed by electron microscopy. The quaternary structure of the P-450LM4 was similar to that demonstrated earlier for P-450LM2.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020298055ZK.pdf | 427KB | download |