Cytochrome P‐450: hexameric structure of the purified LM4 form

FEBS Letters

Myasoedova, K.N.¹  Tsuprun, V.L.² 

[1] N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow 117977, Russian Federation;A.V. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Moscow 117333, Russian Federation

关键词: Cytochrome P-450LM4;    Quaternary structure;    Hexamer;    Immobilized protein;   

DOI  :  10.1016/0014-5793(93)81083-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified cytochrome P-450_LM4 was found to be monodisperse in 20% glycerol by analytical ultracentrifugation. Its S _20,w value was quite similar to that of hexameric P-450_LM2. At lower glycerol concentrations the P-450_LM4 oligomers showed a tendency to aggregate. The P-450_LM4 oligomers were immobilized on Ultrogel A4 under conditions allowing only one covalent link to the matrix per oligomer. In the presence of SDS, the oligomers dissociated leaving only 1/6th of the initial amount of bound protein on the matrix, suggesting that the purified P-450_LM4 is a hexamer. This was confirmed by electron microscopy. The quaternary structure of the P-450_LM4 was similar to that demonstrated earlier for P-450_LM2.

【 授权许可】

Unknown