Involvement of protein dynamics in enzyme stability

FEBS Letters

Glandières, Jean Marie¹  Alpert, Bernard¹  Haouz, Ahmed¹ 

[1] Laboratoire de Biologie Physico-Chimique, Université Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France

关键词: Protein dynamics;    Glucose oxidase;    Immobilized protein;    Thermostability;    Fluorescence quenching;   

DOI  :  10.1016/S0014-5793(01)02917-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dynamics of glucose oxidase immobilized and in solution were compared through their tryptophan fluorescence spectra, decay times and quenching by acrylamide. Energy barrier for thermal inactivation and melting temperature of both soluble and immobilized enzyme were also measured. Data show that the fluctuation amplitude is at the origin of protein instability.

【 授权许可】

Unknown