Bile salt‐stimulated lipase in human milk

FEBS Letters

Bläckberg, Lars¹  Hernell, Olle¹ 

[1] Department of Medical Biochemistry and Biophysics, and Department of Pediatrics, University of Umeå, S-901 87 Umeå, Sweden

关键词: Bile salt-stimulated lipase;    Human milk;    Bile salt;    Lipase;    Enzyme activation;   

DOI  :  10.1016/0014-5793(93)81340-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human milk bile salt-stimulated lipase ensures efficient triacylglycerol utilization in breast-fed newborns. For activity against long-chain triacylglycerol, primary bile salts are a prerequisite. Bile salts also protect the enzyme from inactivation by intestinal proteases. We have studied the effect of different bile salts on activation, protease protection, lipid binding, and enzyme inactivation, caused by an arginine modifying agent. Based on the results we propose a model involving two bile salt binding sites; one activation-site specific for primary bile salt, and another, less specific, lipid binding promoting site at which also secondary bile salt binds. Binding to this latter site induces binding of enzyme to emulsified substrates but without subsequent lipolysis.

【 授权许可】

Unknown