Studies in bile salt solutions

FEBS Letters

Wallace, Robert G.¹  O'Connor, Charmian J.¹ 

[1] Department of Chemistry, University of Auckland, Private Bag, Auckland, New Zealand

关键词: Bile salt-stimulated lipase;    Human milk lipase;    Esterase activity;   

DOI  :  10.1016/0014-5793(84)81347-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of human milk lipase by deoxycholate and its taurine and glycine conjugates was demonstrated by measuring the esterolysis reaction of 4-nitrophenylacetate. The steroidal surfactants did not bind strongly to the polar substrate but they did bind effectively to a hydrophobic site on the enzyme and these bile salt-enzyme complexes were effective catalysts. These results are compared with those for stimulation of the enzyme by cholate surfactants and it has been demonstrated that the absence of a 7α-OH substituent on the steriod nucleus does not prevent stimulation of either the esterolytic or lipolytic activity of the enzyme.

【 授权许可】

Unknown