Isolation of two members of the rat MAP kinase kinase gene family

FEBS Letters

Otsu, Masayuki¹  Terada, Yasuhiko¹  Okayama, Hiroto¹ 

[1] The Okayama Cell Switching Project, Research and Development Corporation of Japan (JRDC), Pasteur Building 4 F, 103-5 Tanaka-monzencho, Sakyo-ku, Kyoto, 606, Japan

关键词: Signal transduction;    MAP kinase kinase;    Molecular cloning;    MAP;    Mitogen activated protein;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)80596-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of 4̃5 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK. may form a large gene family.

【 授权许可】

Unknown