Interleukin 1 and tumour necrosis factor activate the mitogen‐activated protein (MAP) kinase kinase in cultured cells

FEBS Letters

Kracht, Michael¹  Saklatvala, Jeremy¹  Rawlinson, Lesley M.¹  Marshall, Christopher J.² 

[1] Cytokine Biochemistry Department, Strangeways Research Laboratory, Worts' Causeway, Cambridge, CB1 4RN, UK;Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK

关键词: Interleukin 1;    Tumour necrosis factor;    MAP kinase;    MAP kinase kinase;    ERK;    extracellularly regulated kinase;    IL;    interleukin;    MAP;    mitogen-activated protein;    MBP;    myelin basic protein;    PMA;    phorbol myristate acctate;   

DOI  :  10.1016/0014-5793(93)81709-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin 1 (IL1) activated mitogen-activated protein (MAP) kinase kinase in human gingival and foreskin fibroblasts and KB cells. Maximal activity was found in cytosolic extracts made after stimulating cells for 15 min. On anion-exchange chromatography two differently charged forms of MAP kinase kinase were identified, both phosphorylated a kinase-defective mutant MAP kinase, and activated recombinant wild type MAP kinase to phosphorylate MBP. Both were inhibited by an antiserum to recombinant MAP kinase kinase and the less acidic form was identified on Western blotting as an antigen of ∼43 kDa. Indistinguishable forms were very much more strongly induced by phorbol myristate acetate (PMA). TNF had a similar effect to that of IL1.

【 授权许可】

Unknown