Distribution of non‐class‐III β‐tubulin isoforms in neuronal and non‐neuronal cells

FEBS Letters

Linhartová, Irena¹  Viklický, Vladimír¹  Dráber, Pavel¹  Dráberová, Eduarda¹ 

[1]Institute of Molecular Genetics, Czech Academy of Sciences, 142 20 Praha 4, Czech Republic

关键词: Isoelectric focusing;    Post-translational modification;    Subtilisin;    Tubulin isotype;    HRIF;    high-resolution isoelectric focusing;    mAb(s);    monoclonal antibody(ies);    MTP(s);    microtubule protein(s);    SDSPAGE;    sodium dodecylsulphate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(93)81662-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

β-Tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. Post-translational modifications of brain non-class-III β-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. Less extensive modifications were also observed in Neuro-2a, HeLa and 3T3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. The data suggest post-translational modification of non-class-III β-tubulin isoforms in neuronal as well as in non-neuronal cells. Such modification might modulate interaction of tubulin isoforms with microtubule-associated proteins.

【 授权许可】

Unknown