Identification of the phosphorylated β‐tubulin isotype in differentiated neuroblastoma cells

FEBS Letters

Little, Melvyn¹  Zimmermann, Hans-Peter¹  Ludueña, Richard F.² 

[1] Institute for Cell and Tumor Biology, German Cancer Research Center, D-6900 Heidelberg, FRG;Department of Biochemistry, University of Texas, Health Science Center at San Antonio, San Antonio, TX 78284, USA

关键词: β-Tubulin;    Tubulin isotype;    Tubulin phosphorylation;    Neuroblastoma tubulin;   

DOI  :  10.1016/0014-5793(88)80658-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tubulin molecule consists of an α- and a β-subunit, each of which exists in several isotypic forms. It has been previously shown that one of the isotypes of neuroblastoma β-tubulin is phosphorylated at a serine residue in vivo [(1985) J. Cell Biol. 100, 764–774]. Here we identify the phosphorylated isotype as β₂ (type III). Moreover, the large size of the phosphorylated tryptic peptide and sequence comparisons of vertebrate β-tubulins suggest that one of the two serines in positions 444 and 446 is the phosphorylated residue. Our results raise the possibility that β₂-tubulin differs functionally from the other β-tubulin isotypes.

【 授权许可】

Unknown