Kinetics of association and dissociation of colchicine‐tubulin complex from brain and renal tubulin Evidence for the existence of multiple isotypes of tubulin in brain with differential affinity to colchicine

FEBS Letters

Banerjee, Asok¹  Luduena, Richard F.¹ 

[1] Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX 78284-7760, USA

关键词: Colchicine-tubulin;    Biphasic association kinetics;    Biphasic dissociation kinetics;    Fluorescence;    Tubulin isotype;    (Brain;    Kidney);    GTP;    guanosine-5′-triphosphate;    Mes;    2-(N-morpholino)ethanesulfonic acid;    PC-tubulin;    tubulin purified through phosphocellulose chromatography;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(87)81199-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of colchicine binding to bovine brain tubulin have been reported to be biphasic under pseudo first order conditions [(1978) Biochemistry 17, 4466-4472]. Unlike brain tubulin, the kinetics of colchicine binding to bovine renal tubulin are monophasic. The apparent on-rate constant for the binding of colchicine to renal tubulin is found to be very close to that of the faster binding component in brain tubulin. Similarly, the dissociation of colchicine-tubulin complex in the presence of iodide is biphasic for brain tubulin but monophasic for renal tubulin. Since brain and renal tubulin apparently differ in β-tubulin, our results suggest that the biphasic nature of the kinetics for bovine brain tubulin could possibly originate from the existence of multiple isotypes of tubulin differing in drug binding affinity.

【 授权许可】

Unknown