Purification and properties of a 155 kDa bone marrow‐derived glycoprotein enhancing the activity of granulocyte‐macrophage colony stimulating factor (GM‐CSF)

FEBS Letters

Laerum, Ole Didrik¹  Petrov, Rem V.²  Mikhailova, Augusta A.²  Katlinsky, Anton V.¹ 

[1] The Gade Institute, Department of Pathology, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway;Department of Immunology, Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16110, 117871 GSP-7 Moscow V437, Russian Federation

关键词: GM-CSF;    Bone marrow;    Colony formation enhancement;   

DOI  :  10.1016/0014-5793(93)81659-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein has been purified from pig bone marrow which enhances the activity of exogenously added granulocyte-macrophage colony stimulating factor (GM-CSF) on mouse myelopoiEtic colony formation in vitro. The substance alone did not exhibit any colony stimulating activity. The active fraction was isolated from the medium of pig bone marrow suspension cultures during 20–24 h. Purification to homogeneity was then performed by subsequent HPLC and SDS-PAGE. The GM-CSF enhancing protein was identified as a 155 kDa glycoprotein.

【 授权许可】

Unknown