Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture

Revista Brasileira de Otorrinolaringologia

Nascimento, Evaldo¹  Vasconcelos, Anilton César¹  Crosara, Paulo Fernando Tormin Borges¹  Crosara, Sandra Letícia Reis¹  Becker, Celso Gonçalves¹  Universidade Federal de Minas Gerais¹  Guimarães, Roberto Eustáquio Santos¹  Becker, Helena Maria Gonçalves¹ 

关键词: polyposis;    nasal;    eosinophil;    GM-CSF;    IL-5;    mytomicin C.;   

DOI  :  10.1590/S0034-72992005000400010

学科分类：医学（综合）

来源: Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial

【 摘 要 】

The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology. AIM:The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps. STUDY DESIGN: case-control. MATERIAL AND METHOD:This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method. RESULTS:Reduction in GM-CSF expression on the experimental group at time 24 h (p<= 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p=0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours. CONCLUSION:This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.

【 授权许可】

Unknown