A conformation‐dependent monoclonal antibody against active chicken acetylcholinesterase

FEBS Letters

Massoulié, Jean²  Grassi, Jacques¹  Vallette, François-Marie³  Chatel, Jean-Marc² 

[1] Service de Pharmacologie et d'Immunologie, DRIPP, C.E. Saclay, 91191 Gif-sur-Yvette, France;Laboratoire de Neurobíologie, CNRS URA 295, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris, France;Institut de Biologie Physico-chimique, 16 rue P. et M. Curie, 75005 Paris, France

关键词: Acetylcholinesterase: Monoclonal antibody;    Active conformation;    Chicken;   

DOI  :  10.1016/0014-5793(93)80027-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that the C-131 monoclonal antibody, directed against chicken AChE, recognizes active chicken AChE, but not the SDS-denatured or heat-inactivated protein. Previous results indicated that C-131 only binds to the active enzyme, and not to inactive molecules which also occur in the embryonic chicken brain. In contrast with C-131, other monoclonal antibodies obtained in the same series, such as C-6 and C-54, also recognize denatured or inactive AChE. It is noteworthy that these antibodies all seem to react with a trypsin-sensitive peptide which is present in chicken but not in mammalian or Torpedo AChE, whereas the C-131 antibody binds trypsin-modified as well as intact molecules. These results show that C-131 is highly conformation-dependent, specific for active AChE. They confirm our previous conclusion that active and inactive molecules arise from different folding processes.

【 授权许可】

Unknown