A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs

FEBS Letters

Hawkins, Anita L.²  Edgar, Stephanie R.³  Uhl, George R.³  Surratt, Christopher K.³  Griffin, Constance A.²  Li, Xiang¹  Yang, Xiao-Dong³  Persico, Antonio M.³  Jabs, Ethylin W.¹  Bird, Geoffrey S.³ 

[1] Pediatrics and Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA;Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA;Molecular Neurobiology, Addiction Research Center, NIDA, NIH, and Departments of Neurology, Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

关键词: Monoamine transporter;    Chromosomal mapping;    Polymorphism;    N-linked glycosylation;   

DOI  :  10.1016/0014-5793(93)80539-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat sequence, but displays one less consensus site for asparagine N-linked glycosylation and one more consensus site for phosphorylation by protein kinase C. The human SVMT gene maps to chromosome 10q25 using Southern blotting analysis of human/rodent hybrid cell lines and fluorescent in situ hybridization approaches. The cDNA, and a subclone, recognize TaqI polymorphisms that may prove useful to assess this gene's involvement in neuropsychiatric disorders involving monoaminergic brain systems.

【 授权许可】

Unknown