Transport of prepro‐albumin into inverted vesicles prepared from the inner membrane of rat liver mitochondria

FEBS Letters

Tuboi, Syozo¹  Ono, Hideyu¹  Yoshida, Tadashi¹ 

[1] Department of Biochemistry, Yamagata University School of Medicine, Yamagata 990-23, Japan

关键词: Mitochondria;    Protein transport;    Transport machinery;    Re-translocation;    preproRSA;    prepro-rat serum alubumin;    proRSA;    pro-rat serum albumin;   

DOI  :  10.1016/0014-5793(93)80527-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When inverted vesicles prepared from the inner membrane of rat liver mitochondria were incubated with prepro-rat serum albumin, considerable amounts of prepro-albumin and pro-albumin were recovered with the inverted vesicles re-isolated by centrifugation. Pro-albumin was resistant to trypsin, but prepro-albumin was completely digested by trypsin, indicating that prepro-albumin was transported into the vesicles and concomitantly converted to pro-albumin. This transport process required ATP, but not a membrane potential. These results suggest that some export machinery for a protein having an amino acid sequence in its N-terminal portion similar to the signal sequence of secretory protein exists in the inner mitochondrial membrane.

【 授权许可】

Unknown