FEBS Letters | |
Transport of prepro‐albumin into inverted vesicles prepared from the inner membrane of rat liver mitochondria | |
Tuboi, Syozo1  Ono, Hideyu1  Yoshida, Tadashi1  | |
[1] Department of Biochemistry, Yamagata University School of Medicine, Yamagata 990-23, Japan | |
关键词: Mitochondria; Protein transport; Transport machinery; Re-translocation; preproRSA; prepro-rat serum alubumin; proRSA; pro-rat serum albumin; | |
DOI : 10.1016/0014-5793(93)80527-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
When inverted vesicles prepared from the inner membrane of rat liver mitochondria were incubated with prepro-rat serum albumin, considerable amounts of prepro-albumin and pro-albumin were recovered with the inverted vesicles re-isolated by centrifugation. Pro-albumin was resistant to trypsin, but prepro-albumin was completely digested by trypsin, indicating that prepro-albumin was transported into the vesicles and concomitantly converted to pro-albumin. This transport process required ATP, but not a membrane potential. These results suggest that some export machinery for a protein having an amino acid sequence in its N-terminal portion similar to the signal sequence of secretory protein exists in the inner mitochondrial membrane.
【 授权许可】
Unknown
【 预 览 】
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