Fusidic acid‐dependent ribosomal complexes protect Escherichia coli ribosomes from the action of the type 1 ribosome‐inactivating protein crotin 2

FEBS Letters

Alegre, Carlos²  Girbés, Tomás²  Escarmis, Cristina¹  Iglesias, Rosario²  Ferreras, J.Miguel² 

[1] Centra de Biologia Molecular (CSIC-UAM), Canto Blanco, Madrid, Spain;Departamento de Bioquimlca y Biologia Molecular, Facultadde Ciencias, Universidad de Valladolid, E-47005 Valladolid, Spain

关键词: Ribosome-inactivating protein;    Escherichia coli;    Protein synthesis;    Fusidic acid;    23 S rRNA from E. coli;   

DOI  :  10.1016/0014-5793(93)80019-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The type 1 ribosome-inactivating protein crotin 2 depurinated Escherichia coli ribosomes which, upon treatment of the isolated rRNA with acid aniline, released a fragment of around 240 nucleotides whose 5'-end sequence was 5'-GAGGACCGGAGUGGAC-3'. The formation of fusidic acid-dependent ribosomal complexes completely prevented release of the fragment. Ribosomes from crotin 2-pretreated fusidic acid complexes were insensitive to acid aniline. They released the RNA fragment only after a second treatment with crotin 2 and acid aniline whereas unprotected ribosomes released the fragment directly after acid aniline.

【 授权许可】

Unknown