Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor

FEBS Letters

Kaghad, M.¹  Ferrara, P.²  Chalon, P.¹  Vita, N.²  Gully, D.³  Dumont, X.¹  Lefort, S.²  Caput, D.¹  Laurent, P.¹  Le Fur, G.³ 

[1]Unité Biologie Moléculaire du Gène, Sanofi Elf Biorecherches, BP 137, 31676 Labège Cedex, France

[2]Unité Biochimie des Protéines, Sanofi Elf Biorecherches, BP 137, 31676 Labège Cedex, France

[3]Sanofi Recherche, 195 route d'Espagne, 31036 Toulouse Cedex, France

关键词: Neurotensin;    Receptor;    Cloning;    Binding;    HT 29 cell;   

DOI  :  10.1016/0014-5793(93)81509-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847-854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.

【 授权许可】

Unknown