Identification and expression of a variant isoform of the levocabastine‐sensitive neurotensin receptor in the mouse central nervous system

FEBS Letters

Vincent, Jean-Pierre¹  Botto, Jean-Marie¹  Mazella, Jean¹  Sarret, Philippe¹ 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UPR 411, 660 Route des Lucioles, 06560 Valbonne, France

关键词: Neurotensin;    Receptor;    Low affinity;    Levocabastine;    Variant;    Cloning;    bp;    base pair;    NT;    neurotensin;    NTR;    neurotensin receptor;    NTRH;    high-affinity neurotensin receptor;    NTRL;    low-affinity neurotensin receptor;    nfNTRL;    non-functional low-affinity neurotensin receptor;    SSC;    sodiumsodium citrate;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(96)01391-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This work describes the molecular cloning of a variant isoform of the low-affinity levocabastine-sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS-7 cells, this non-functional neurotensin receptor is expressed in cerebral neocortex, cerebellum, olfactory bulb, striatum and hypothalamus with a level similar to that of the full-length low-affinity neurotensin receptor. By contrast, this receptor form is very weakly expressed in mesencephalon and absent in the pituitary, but is the major product in the spinal cord.

【 授权许可】

Unknown