Evidence for a role for protein kinase C in the stimulation of protein synthesis by insulin in swiss 3T3 fibroblasts

FEBS Letters

Welsh, Gavin I.¹  Proud, Christopher G.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK

关键词: Insulin;    Protein synthesis;    Protein kinase C;    eIF-;    eukaryotic initiation factor;    PKC;    protein kinase C;    PDBu;    phorbol dibutyrate;   

DOI  :  10.1016/0014-5793(93)81300-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the role of protein kinase C (PKC) in the stimulation of protein synthesis by insulin using two complementary approaches. In the first, fibroblasts were pretreated with phorbol esters to down-regulate PKC. In these cells, the effects of insulin and of phorbol esters on protein synthesis were completely abolished, although serum still elicited an effect approaching that seen in control cells. Secondly, we used newly developed inhibitors of PKC which, again, blocked the effects of insulin and phorbol esters without greatly reducing the response to serum. Thus PKC apparently plays an important role in the stimulation of translation by insulin.

【 授权许可】

Unknown