Altered response to growth factors in rat epithelial liver cells overexpressing human c‐Fos protein

FEBS Letters

Martel, P.¹  Seillan-Heberden, C.¹  Gaillard-Sanchez, I.¹  Lagarrigue, S.¹ 

[1] Laboratoire de Nutrition et de Sécurité Alimentaire, INRA, 78352 Jouy en Josas Cedex, France

关键词: c-fos;    TGFβ;    EGF;    Rat liver epithelial cell;   

DOI  :  10.1016/0014-5793(92)81514-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human c-fos cDNA was transfected into normal rat liver epithelial (REL) cells to identify cellular modifications associated with high expression of c-Fos protein. Responses to EGF and TGFβ were examined in the different cell lines, under anchorage-dependent and -independent conditions. Sensitivity to both factors was modified in transfected cells. While parental cells in monolayer did not respond to EGF, c-fos containing cells growth was stimulated by this factor. Overexpression of c-Fos protein led to an enhanced TGFβ-induced growth inhibition under anchorage dependent conditions, and TGFβ abolished spontaneous growth in soft agar of the cell lines containing c-fos oncogene. The mechanisms underlying, the increased sensitivity to TGFβ in c-fos transfected cells are still to be determined.

【 授权许可】

Unknown