The role of protein tyrosine phosphatases in density‐dependent growth control of normal rat kidney cells

FEBS Letters

Völler, Maureen C.W.²  van Zoelen, Everardus J.J.²  Rijksen, Gert¹ 

[1] Department of Hematology, Laboratory of Medical Enzymology, University Hospital, Utrecht, The Netherlands;Department of Cell Biology, University of Nijmegen, Nijmegen, The Netherlands

关键词: Density-dependent growth inhibition;    Normal rat kidney cell;    Protein tyrosine phosphatase;    Retinoic acid;    Transforming growth factor-β;    Vanadate;    EGF;    epidermal growth factor;    NRK;    normal rat kidney;    PDGP;    platelet-derived growth factor;    PTPase;    protein tyrosine phosphatase;    RA;    retinoic acid;    TGFβ;    transforming growth factor-β;   

DOI  :  10.1016/0014-5793(93)81117-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In normal rat kidney cells protein tyrosine phosphatases (PTPases) play a role in attaining density-dependent growth arrest after stimulation with mitogens. The PTPase inhibitor sodium orthovanadate prevents density-dependent growth inhibition of EGF-treated cells and mimicks in that respect the action of TGFβ and retinoic acid. However, enhanced PTPase activity is not obligatory for maintaining cells in a density-arrested state. In contrast to TGFβ and retinoic acid, vanadate is unable to restimulate density-inhibited cells, indicating that different mechanisms are operating. Yet, vanadate is strongly potentiating the effect of low concentrations of TGFβ but not of retinoic acid, implicating that tyrosine phosphorylation is linked to TGFβ action and that PTPase may represent a negative control element in the TGFβ signaling pathway.

【 授权许可】

Unknown