The membrane proximal region of the cytoplasmic domain of the growth hormone receptor is involved in the activation of Stat 3

FEBS Letters

Kelly, Paul A.¹  Moutoussamy, Soraya¹  Binart, Nadine¹  Sotiropoulos, Athanassia¹  Finidori, Joëlle¹ 

[1] INSERM U344, Faculté de Médicine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France

关键词: Growth hormone;    c-fos;    Stat 3;    Cytokine receptor;    GH;    growth hormone;    hGH;    human growth hormone;    GHR;    growth hormone receptor;    Jak;    Janus kinases;    Stat;    signal transducer and activator of transcription;    SIE;    c-sis-inducible element;    SRE;    serum-inducible element;    SIF;    c-sis-inducible factor;    IFN;    interferon;    GAS;    IFNγ activation site;    PRL;    prolactin;    EGF;    epidermal growth factor;    PDGF;    platelet derived growth factor;    LIF;    leukaemia inhibitory factor;    GM-CSF;    granulocyte-macrophage colony-stimulating factor;    p-Tyr;    phosphotyrosine;   

DOI  :  10.1016/0014-5793(95)00734-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Growth hormone receptor (GHR) signaling involves activation of the Janus Kinases (Jak) and of Stat proteins (signal transducers and activators of transcription). Growth hormone (GH) induces transcriptional activation of c-fos gene and the c-sis inducible element (SIE) of its promoter was shown to bind the Stat proteins. Using cells co-transfected with GHR and Stat 3 expression vectors, we directly demonstrate that GH induces tyrosine phosphorylation of Stat 3 and its binding to the SIE probe. We showed, using mutant forms of GHR, that only the cytoplasmic membrane proximal domain of the receptor, including a conserved proline rich region (box 1), is required for this effect.

【 授权许可】

Unknown