The amino acid sequence of a type I copper protein with an unusual serine‐ and hydroxyproline‐rich C‐terminal domain isolated from cucumber peelings

FEBS Letters

Mann, Karlheinz²  Thoenes, Ulrich²  Schäfer, Wolfram²  Mehrabian, Zara¹  Messerschmidt, Albrecht²  Nalbandyan, Robert¹ 

[1] Institute of Biochemistry, Academy of Sciences of the Armenian Republic, 375044 Yerevan, Armenia;Max-Planck-Institut für Biochemie, 8033 Martinsried, Germany

关键词: Amino acid sequence;    Copper protein;    Stellacyanin;    Pyroglutamate;    4-Hydroxyproline;   

DOI  :  10.1016/0014-5793(92)81475-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have determined the amino acid sequence of a small copper protein isolated from cucumber peelings. This cupredoxin contains 137 amino acids including a pyroglutamate as the first residue. The N-terminal 110 amino acid-long domain shows 30–37% identity to 2 other cupredoxins, stellacyanin and cucumber basic blue protein. A unique feature of this protein is a 27 amino acid-long, C-terminal domain rich in 4-hydroxyproline and serine and resembling certain plant cell wall proteins. The prolines in this domain are hydroxylated to a different extent depending on the surrounding sequence.

【 授权许可】

Unknown