Reconstitution of stellacyanin as a case of direct Cu(I) transfer between yeast copper thionein and ‘blue’ copper apoprotein

FEBS Letters

Hartmann, Hans-Jürgen¹  Weser, Ulrich¹  Rotilio, Giuseppe²  Desideri, Alessandro²  Morpurgo, Laura² 

[1] Anorganische Biochemie, Physiologisch-chemisches Institut der Universität Tübingen, 7400 Tübingen, FRG;Istituto di Chimica Biologica e Centro di Biologia Molecolare del C.N.R., Università di Roma, Roma, Italy

关键词: Metallothionein;    Yeast Cu-thionein;    Stellacyanin;    Cu(I)-transfer;    Copper;   

DOI  :  10.1016/0014-5793(83)80489-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of ‘blue’ apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu-thionein, without any detectable unspecific Cu(II) intermediate, both aerobically and anaerobically.

【 授权许可】

Unknown