Cross‐linking of apolipoproteins is involved in a loss of the ligand activity of high density lipoprotein upon Cu2+‐mediated oxidation

FEBS Letters

Sakamoto, Yu-Ichiro¹  Sakai, Masakazu²  Shichiri, Motoaki²  Horiuchi, Seikoh¹  Miyazaki, Akira¹ 

[1]Department of Biochemistry, Kumamoto University School of Medicine, Kumamoto, Japan

[2]Metabolic Medicine, Kumamoto University School of Medicine, Kumamoto, Japan

关键词: High density lipoprotein;    Apolipoprotein;    Chemical modification;    Cholesterol efflux;    Oxidation;    HDL;    high density lipoprotein;    TNM;    tetranitromethane;    TNM-HDL;    TNM-treated HDL;    LDL;    low density lipoprotein;    acetyl-LDL;    acetylated LDL;    TBARS;    thiobarbituric acid reactive substances;    CE;    cholesteryl esters;   

DOI  :  10.1016/0014-5793(92)80974-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recent study demonstrated that Cu²⁺-mediated oxidation of high density lipoprotein (HDL) resulted in a loss of the capacity to reduce cholesterol from macrophage foam cells [(1991) Proc. Natl. Acad. Sci. USA 88, 6457–6461]. In the present study we characterized the physicochemical properties of oxidized HDL and correlated them with the ligand activity toward the HDL receptor. Among them, the cross-linking of apolipoproteins and an increase in lipid peroxides were characteristic and closely similar to those of tetranitromethane-treated HDL, an abortive ligand for the HDL receptor. Cellular experiments with murine peritoneal macrophages revealed that both the cellular binding activity of HDL and its capacity to enhance cholesterol efflux from macrophage foam cells were markedly reduced upon oxidation. These results suggest that cross-linking of HDL apolipoproteins is involved in the loss of the ligand activity of oxidized HDL.

【 授权许可】

Unknown