A GC box in the bidirectional promoter is essential for expression of the human dihydrofolate reductase and mismatch repair protein 1 genes

FEBS Letters

Fujii, Hiroyuki¹  Shinya, Eiji¹  Shimada, Takashi¹ 

[1] Clinical Hematology Branch, National Heart Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Dihydrofolate reductase;    Bidirectional promoter;    Luciferase;    Transcription control;    GC box;    TATA box;    DHFR;    dihydrofolate reductase;    MRPI;    mismatch repair protein 1;    CAT;    chloramphenicol acetyltranferase;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(92)81455-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human dehydrofolate reductase and mismatch repair protein 1 genes are organized in a head-to-head configuration separated by an 88 base-pair segment and directed by a bidirectional promoter. In vivo transient assays of the site directed mutant promoters using firefly luciferase as a reporter showed that an AT-rich sequence, ACAAATA, in the GC-rich promoter sequence is not required for transcription. However, two out of four GC boxes were shown to function as bidirectional positive regulatory elements. Among them, a GC box at the midpoint of the region between the two initiation sites is essential for supporting minimal bidirectional activity.

【 授权许可】

Unknown