FEBS Letters | |
A GC box in the bidirectional promoter is essential for expression of the human dihydrofolate reductase and mismatch repair protein 1 genes | |
Fujii, Hiroyuki1  Shinya, Eiji1  Shimada, Takashi1  | |
[1] Clinical Hematology Branch, National Heart Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA | |
关键词: Dihydrofolate reductase; Bidirectional promoter; Luciferase; Transcription control; GC box; TATA box; DHFR; dihydrofolate reductase; MRPI; mismatch repair protein 1; CAT; chloramphenicol acetyltranferase; bp; base pairs; | |
DOI : 10.1016/0014-5793(92)81455-U | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The human dehydrofolate reductase and mismatch repair protein 1 genes are organized in a head-to-head configuration separated by an 88 base-pair segment and directed by a bidirectional promoter. In vivo transient assays of the site directed mutant promoters using firefly luciferase as a reporter showed that an AT-rich sequence, ACAAATA, in the GC-rich promoter sequence is not required for transcription. However, two out of four GC boxes were shown to function as bidirectional positive regulatory elements. Among them, a GC box at the midpoint of the region between the two initiation sites is essential for supporting minimal bidirectional activity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020297177ZK.pdf | 442KB | download |