ATP‐activated Ca2+‐permeable channels in rat peritoneal macrophages

FEBS Letters

Naumov, Alexander P.¹  Kuryshev, Yuri A.¹  Kaznacheyeva, Elena V.¹  Mozhayeva, Galina N.¹ 

[1] Institute of Cytology of Russian Academy of Sciences, 194064 St. Petersburg, Russian Federation

关键词: Macrophage;    ATP;    Patch clamp;    Calcium channel;    ATP;    adenosine triphosphate;    DMEM;    Dulbecco's modified Eagle's medium;    EGTA;    ethyleneglycol-bis-(β-aminoethyl ether)-N;    N′-tetraacetic acid;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid;    GTP;    guanosine triphosphate;    GTPγS;    5′-O-(3-thiotriphosphate);   

DOI  :  10.1016/0014-5793(92)81210-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The patch-clamp technique was used to study mechanisms of ATP-induced Ca²⁺ influx in rat peritoneal macrophages. The experiments on whole-cell and patch membranes have shown that extracellular ATP activates channels permeable to di- and monovalent inorganic cations. Ratios of unitary channel conductances in 105 mM Ca²⁺, Sr²⁺, Mn²⁺, Ba²⁺ and normal sodium solutions were 1.0, 0.95, 0.75, 0.55 and 0.85, respectively. The channels could open in the presence of non-hydrolyzable GTP analogues in artificial intracellular solution. The data are consistent with the hypothesis that a GTP-binding protein is involved in receptor-to-channel coupling.

【 授权许可】

Unknown