The voltage‐gated Ca2+ release channel in the vacuolar membrane of sugar beet resides in two activity states

FEBS Letters

Sanders, Dale¹  Johannes, Eva¹ 

[1] The Plant Laboratory, Biology Department, University of York, PO Box 373, York, YO1 5YW, UK

关键词: Calcium channel;    Signal transduction;    Patch clamp;    Vacuole;    Beta vulgaris;   

DOI  :  10.1016/0014-5793(95)00424-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The voltage-gated Ca²⁺ release channel in the vacuolar membrane of sugar beet tap roots resides in two states which show similar reversal potentials in biionic conditions but differ dramatically in their single channel current and open frequency. State I has a unitary Ca²⁺ conductance of 3.9 ± 0.5 pS and channel openings are rare at vacuolar resting potentials. After spontaneous and reversible transition into State II the unitary Ca²⁺ conductance increases three-fold, accompanied by a concomitant rise in channel activity over the physiological voltage range. The 15- to 19-fold increase in total Ca²⁺ current after the State I to State II transition could play an essential role in channel activation during signal transduction.

【 授权许可】

Unknown