Inositol 1,4,5‐trisphosphate activates two types of Ca2+‐permeable channels in human carcinoma cells

FEBS Letters

Naumov, Alexander P.¹  Kuryshev, Yuri A.¹  Mozhayeva, Galina N.¹ 

[1] Institute of Cytology, Academy of Sciences of the USSR, Leningrad, USSR

关键词: Patch clamp;    Carcinoma cell;    Calcium channel;    Epidermal growth factor;    Inositol 1;    4;    5-trisphosphate;    IP3;    inositol 1;    4;    5-trisphosphate;    GTP-γS;    guanosine 5'-O-(3-thiotriphosphate);    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    EGTA;    ethylene glycol-bis β-amino-ethylether) N;    N;    N';    N'-tetraacetic acid;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(90)80853-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ca²⁺-permeable channels in human carcinoma A431 cells were studied using the patch clamp technique. We have found two types of Ca²⁺-penneable channels which are activated by inositol 1,4,5-trisphosphate (IP₃) applied to the intracellular side of the plasma membrane. Unitary conductances of these channels are 3.7 and 13 pS (105 mM Ca²⁺ in recording pipette, 30–33°C). From the extracellular side of the membrane the channels are activated by EGF. It is assumed that extracellular agonists open both channel types by stimulating the release of IP₃ from the membrane.

【 授权许可】

Unknown