Molluscan putative prohormore convertases: Structural diversity in the central nervous system of Lymnaea stagnalis

FEBS Letters

Smit, August B.¹  Spijker, Sabine¹  Geraerts, Wijnand P.M.¹ 

[1]Department of Biology, Vrije, Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

关键词: PC2-related convertase;    Furin-related convertases;    cDNA cloning;    Polymerase chain reaction;    Cerebral ganglia;    Lymnaea stagnalis;   

DOI  :  10.1016/0014-5793(92)80938-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the cerebral ganglia of the central nervous system of the freshwater snail Lymnaea stagnalis many neuropeptides are proteolytically processed from larger prohormones at sites marked by both single and multiple basic amino acids. In the present study we identified cloned cDNA and PCR products corresponding to three putative endoproteases that may be involved in prohormone processing. The cDNA encodes a protein of 653 residues with an overall sequence identity of 60%, 41%, 35%, 40%, and 27% with the recently characterized endoproteases PC2, PC1/3, PC4, furin and Kex2, respectively. The Lymnaea preproconvertase has ∼80% homology with the catalytic domain, and ∼40% and 5̃0 with the N-and C-terminal part, respectively, of the vertebrate PC2. Two cloned PCR products, Lfur 1 and Lfur 2, show highest sequence identity to furin. Expression of the LPC2 gene is exclusively in the central nervous system, where two LPC2 transcripts of 3.0 and 4.8 kb were detected.

【 授权许可】

Unknown