cDNA cloning of a new type of subunit of mammalian proteasomes

FEBS Letters

Balson, Deborah F.¹  Thomson, Stuart¹  Rivett, A.Jennifer¹ 

[1] Department of Biochemistry, University of Leicester, Leicester, UK

关键词: Proteasome;    Multicatalytic proteinase;    Subunit RN3;    cDNA cloning;    Polymerase chain reaction;    PAGE;    polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;    RSV;    Rous sarcoma virus;   

DOI  :  10.1016/0014-5793(93)81553-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The primary structure of a new type of subunit (RN3) of rat proteasomes (multicatalytic proteinase complexes) has been determined from the nucleotide sequence of the cDNA. The cDNA encodes a protein of 232 amino acids but the directly determined N-terminal amino acid sequence suggests that the subunit is post-translationally processed to a M _r = 24k form. Sequence alignments reveal a similarity of RN3 to other proteasome subunits. It can be designated a B-type proteasomal subunit but is not closely related to the β subunit of the archaebacterial proteinase or to other members of the B group.

【 授权许可】

Unknown