A voltage‐gated calcium channel is linked to the antigen receptor in Jurkat T lymphocytes

FEBS Letters

Szabo, Gabor²  Gray, Lloyd S.¹  Densmore, John J.¹ 

[1] Interdisciplinary Graduate Program in Biophysics, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA;Department of Physiology, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA

关键词: Electrophysiology;    T lymphocyte;    Calcium channel;    Activation;    Antigen receptor;   

DOI  :  10.1016/0014-5793(92)80926-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of T lymphocytes results in an increase in intracellular Ca²⁺ due in large part to influx of extracellular Ca²⁺. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of −90 mV but not from −60 mV. This whole-cell current was insensitive to tetrodotoxin, carried by Ba²⁺, and blocked by Ni²⁺. Occupancy of the T lymphocyte antigen receptor increased the current's magnitude. These data suggest that antigen receptor-induced Ca²⁺ entry in T lymphocytes may be mediated by a voltage-regulated Ca channel.

【 授权许可】

Unknown