The thyroid hormone response element is required for activation of the growth hormone gene promoter by nicotinamide analogs

FEBS Letters

Aranda, Ana¹  Sánchez-Pacheco, Aurora¹ 

[1] Instituto de Investigaciones Biomédicas, CSIC, Madrid, Spain

关键词: Triiodothyronine;    Nicotinamide;    c-erbaA;    Growth hormone promoter;    GH;    growth hormone;    T3;    triiodothyronine;    TRE;    thyroid hormone response element;   

DOI  :  10.1016/0014-5793(92)81406-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N′-Methylnicotinamide and nicotinamide, which decreased in vitro ADP-ribosylation of nuclear proteins and/or cellular NAD⁺ content, selectively increased the basal expression of the rat growth hormone (GH) gene promoter and its response to triiodothyronine (T3). This increase was not found when the thyroid hormone response element (TRE) was deleted from the promoter. Transfection with an expression vector for the T3 receptor inhibited basal activity of the TRE-containing promoter and repressed the stimulatory effect of N′-methylnicotinamide. The addition of hormone relieved this inhibition and enhanced transcription above levels found in the absence of the transfected receptors. These results suggest a modulatory role of ADP-ribosylation in hormonal regulation of gene expression.

【 授权许可】

Unknown