Identification and endothelin‐induced activation of multiple extracellular signal‐regulated kinases in aortic smooth muscle cells

FEBS Letters

Suga, Shin-ichi¹  Imura, Hiroo¹  Nakao, Kazuwa¹  Yoshimasa, Takaaki¹  Kishimoto, Ichiro¹  Kiso, Yoshiaki² 

[1] Second Division, Department of Medicine, Kyoto University School of Medicine, Kyoto 606, Japan;Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Kyoto 607, Japan

关键词: Endothelin;    Endothelin receptor;    Aortic smooth muscle cell;    Extracellular signal-regulated kinase;    MBP/MAP2 kinase;    Cell proliferation;    ET;    endothelin;    ETR;    endothelin receptor;    SMC;    smooth muscle cell;    MAP2;    microtubule-associated protein 2;    MAP;    mitogen-activated protein;    MBP;    myelin basic protein;    ERK;    extracellular signal-regulated kinase;   

DOI  :  10.1016/0014-5793(92)81100-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to explore intracellular signaling pathways of the mitogenic action of endolhelin (ET), we examined the effect of ET on activities of extracellular signal-regulated kinases (ERKS) in rat aortic smooth muscle cells (SMCs). Treatment of rat aortic SMCs with ET-1 increased kinase activities toward myelin basic protein (MBP). Both 43- and 41-kDa proteins were activated when kinase assays were done in MBP-containing polyacrylamide gels after SDS-PAGE. These proteins were identified as ERK1 and ERK2 with immunoprecipitation and immunoblotting using antipeptide antibodies, respectively. These results indicate that ERKs mediate signal transduction by ET.

【 授权许可】

Unknown