Transforming growth factor‐β1 rapidly activates phosphorylase in a calcium‐dependent manner in rat hepatocytes

FEBS Letters

Hahm, Sung Ho¹  Cooper, Ronald H.¹ 

[1] Department of Pharmacology, University of South Carolina School of Medicine, Columbia, SC 29208, USA

关键词: Transforming growth factor-β1;    Phosphorylase;    Calcium;    Hepatocyte;    Signal transduction;    Growth factor;    TGF-β;    transforming growth factor beta;    [Ca2+]i;    cytosolic free calcium concentration;    indo-1/AM;    1-[2 amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2′-amino-5′-methylphenoxy)ethane-N;    N;    N′;    N′-tetraacetic acid;    penta-acetoxymethylester;   

DOI  :  10.1016/0014-5793(92)81361-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transforming growth factor-β1 (TGF-β1) rapidly activated phosphorylase in isolated rat hepatocytes (half-maximal rate of activation with approximately 0.1 ng/ml). Removal of Ca²⁺ from the external medium just before TGF-β1 addition markedly attenuated phosphorylase activation. TGF-β1 (1 ng/ml) produced a small increase in [Ca²⁺]_i (approximately 10% increase after 30 s), which appears sufficient to account for phosphorylase activation. These observations indicate that activation of the TGF-β1 signal transduction system in hepatocytes is linked with a small increase in [Ca²⁺]_i, and external Ca²⁺ may contribute in part to this increase.

【 授权许可】

Unknown