Evidence for the involvement of carboxyl groups in passive calcium uptake by liver plasma membrane vesicles and in agonist‐induced calcium uptake by hepatocytes

FEBS Letters

Bygrave, Fyfe L.¹  Karjalainen, Ari¹ 

[1] Department of Biochemistry, Faculty of Science, Australian National University, Canberra, ACT 2601, Australia

关键词: Calcium;    Hepatocyte;    Channel;    Carbodiimide;    CMCD;    1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    EDAC;    1-ethyI-3-(3-dimethylaminopropyl)carbodiimide;    EEDQ;    N-ethoxycarbonyl-2-ethoxy-1;    2-hydroquinoline;   

DOI  :  10.1016/0014-5793(89)81141-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydrophobic reagents DCCD and EEDQ, each of which reacts with protein carboxyl groups, were found to inhibit both passive Ca²⁺ uptake by plasma membrane vesicles isolated from rat liver and agonist-induced Ca²⁺ uptake by hepatocytes. The data raise the possibility that the Ca²⁺ inflow pathway(s) in liver has a specific requirement for a reactive carboxyl group or groups.

【 授权许可】

Unknown