Monoclonal antibodies against the presynaptic calcium channel antagonist ω‐conotoxin GVI A from cone snail poison

FEBS Letters

Feuerstein, G.¹  Tombaccini, D.²  Pollard, H.B.²  Adeyemo, O.M.¹ 

[1] Department of Neurology, Uniformed Services University of the Health Sciences, Bethesda, MDUSA;Laboratory of Cell Biology and Genetics, NIDDK, National Institutes of Health, Bethesda, MDUSA

关键词: Conotoxin;    Calcium;    Monoclonal;    Channel;    Presynaptic;    Transport;    ω-ctx GVIA;    ω-conotoxin GVI A;    PBS;    phosphate-buffered saline;    ELISA;    enzyme-linked immunosorbent assay;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(90)80639-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies have been prepared against co-conotoxin GVI A, a peptide isolated from marine snails of the genus Conus (Conus geographus and Conus magus). This toxin is a blocker of select presynaptic Ca²⁺ channels in the central nervous system. Antigenic ω-conotoxin GVI A was synthesized as a covalent conjugate with bovine serum albumin and injected s.c. An ELISA assay combined with a competitive inhibition assay was used to select and characterize monoclonal antibodies able to recognize and bind the free toxin. Several of the antibodies were found to block ω-conotoxin GVI A inhibition of ⁴⁵Ca transport into rat brain synaptosomes and to block ω-conotoxin GVI A binding to membranes from the same preparation. The antibodies recognize native, synthetic toxin, and are useful for analysis of toxin in biological fluids.

【 授权许可】

Unknown