Processing of hepatocyte growth factor to the heterodimeric form is required for biological activity

FEBS Letters

Taylor, William G.¹  Gak, Eva¹  Rubin, Jeffrey S.¹  Chan, Andrew M.-L.¹ 

[1] Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA

关键词: Hepatocyte growth factor;    Scatter factor;    Post-translational modification;    Plasminogen;   

DOI  :  10.1016/0014-5793(92)81356-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatocyte growth factor is a plasminogen-like molecule with diverse biological effects. Although it is synthesized as a single chain polypeptide, it was originally purified as a disulfide-linked haterodimer which was generated by an internal proteolytic event. Subsequent work indicated that preparations consisting largely of the monomeric form also exhibited potent activity. By using a combination of protease inhibition and site-directed mutagenesis, we established that conversion of the single chain polypeptide to the hoterodimer occurred during the bioassay and was required ror mitogenic and motogenic activity.

【 授权许可】

Unknown